from the invisible to the visible word and back

In the world of molecular biology is very important to use specialized techniques to detect proteins one of these is the western blot or protein immunoblot, this technique is used to recognize proteins by means of primary and secondary antibodies, this artificial antibodies are created that react with a specific target protein, the sample to be tested is prepared and put together with these antibodies on a membrane, if the specific protein sought for is present, after gel electrophoresis step this will result in an accordingly stained band in the western blot.
Another important technique is the immunofluorescence is a powerful method to visualize the intracellular processes, conditions and structure that make it in a sample or the antibody that detects a molecule (usually proteins), this carries a molecule fluorescent or well recognized by a Second Antibody bearing this fluoride. Immunofluorescence has traditionally relied on the use of secondary antibodies which have been labeled with fluorescent dyes or fluorescent proteins, however labeled secondary antibodies can be a common source of unwanted background signal. To circumvent this issue, many researchers prefer to instead use directly labeled primary antibodies for detection, but its more expensive.
Personally, I believe that these methods are fundamental for my career, for example in the case that we want to recognize the place or presence of a specific protein in the cell before and after infection with a bacterium.In the present and in the future it is very important to identify how the locations or protein levels vary through an infection, since this way we can account for the effect caused by a bacterium in an X cell and in this way perhaps create something that allows the defense of This cell to some pathological infection.
The immunofluorescence is my favorite technique since, when incubating the anticuepro with the fluorescence, you can choose many different colors that, under the microscope they look very cute